Research overview: We focus on the development, application and integration of high-throughput technologies (including affinity purification of macromolecular complexes, transcriptomics, semi-automated high-throughput microscopy, functional genomics and mass spectrometry-based proteomics) with computational biology to gain systems-level insights into macromolecular dynamics. We have use yeast as a tractable model system to develop these systems biology approaches in the context of cell biology, with a focus on nuclear organization and organelle biogenesis (nuclear pore complex structure/function and peroxisome biogenesis and function) and we apply systems level approaches and concepts in the context of infectious disease.
2. One-cell Doubling Evaluation by Living Arrays of Yeast, ODELAY!
Movie SW1.avi: ODELAY records time laps microscopy of 96 strains grown over 48 hours. Each box is a 3x3mm area of agar imaged in 1/2 hour time intervals. Different strains are observed growing at different rates.
Movie HIS3.avi: Time laps microscopy of a single yeast strain of BY4741 his3∆ demonstrating. Different colony sizes indicate heterogeneity in a genetically identical yeast strain.