Nanobody Production

The NCDIR is actively optimizing the pipeline for the production of nanobodies, highly interesting naturally occurring antibody variants isolated from Camelids. These 15 kDa antibody fragments are isolated from naturally occurring Heavy Chain antibodies (HCAb) made in llamas and alpacas (below is the production pipeline utilized):    

Overview of nanobody identification and production pipeline.
From "A robust pipeline for rapid production of versatile nanobody repertoires" Nat Methods. 2014 Dec;11(12):1253-60