Below is a working list of our current protocols and methodologies.

Please contact us if you have questions or comments, or would like to submit a protocol.

Disruption of Yeast or Bacteria Cells
Harvesting Cells and Making Noodles
Video tutorial Harvesting Cells and Making Noodles
Video tutorial Cryogenic Disruption Using PM100 Ball Mill – grinding.mp4
Cryogenic Disruption of Yeast Cells PM100
Microfluidizer
Zymolase Treatment

Disruption of Mammalian Cells
Cryogenic Disruption of Mammalian Cells using Retsch PM 100
Protein Affinity Capture from Cryogenically Disrupted Human Cells
Biotechniques Paper

Subcellular Fractionation
Yeast Nuclear Envelopes and Nuclear Pore Complex Prep
Yeast Nuclei & Spindle Pole Bodies Prep
Carbonate Extraction of Nuclear Envelopes
Rapid Subcellular Fractionation for Nuclei and Nuclear Envelopes
Heparin Extraction of Nuclear Envelopes

Isolation of Protein Complexes
Conjugation of Tosylactivated Dynabeads
Conjugation of Epoxyactivated Dynabeads
Conjugation of Magnetic Beads
Pullouts with IgG Resin
Immunoaffinity purifications of protein complexes