TR&D Project 2. The Analysis Stage I: Tools for Analyzing the Composition and Stoichiometry of Macromolecular Assemblies
We will develop methods to faithfully and fully identify the bone fide components of any target macromolecular complex in the cell, as well as that of vicinal associators, with high accuracy and throughput.
Our overall goals are to be able to create and optimize methods to:
Goal 1: characterize any assembly in the cell – identifying its individual components
Goal 2: determine how many of those individual components make up the complex – the stoichiometry of the complex
We aim to provide this technology to the community in the form of a “Stoichiometry Package”. This package will incorporate a parallel suite of techniques that give complementary and cross-verifying information, collectively providing accurate, comprehensive sets of stoichiometry data (and dynamic changes) for an isolated assembly. Users can choose any or a combination of approaches, depending on need.