Protocols

Below is a working list of NCDIR protocols and methodologies.

Please contact us if you have questions or comments, or would like to submit a protocol.

Disruption of Yeast or Bacteria Cells
Harvesting Cells and Making Noodles
Harvesting Cells and Making Noodles
Cryogenic Disruption Using PM100 Ball Mill – grinding.mp4
Cryogenic Disruption of Yeast Cells PM100
Microfluidizer
Zymolase Treatment

Disruption of Mammalian Cells
Cryogenic Disruption of Mammalian Cells using Retsch PM 100
Protein Affinity Capture from Cryogenically Disrupted Human Cells

Subcellular Fractionation
Yeast Nuclear Envelopes and Nuclear Pore Complex Prep
Yeast Nuclei & Spindle Pole Bodies Prep
Carbonate Extraction of Nuclear Envelopes
Rapid Subcellular Fractionation for Nuclei and Nuclear Envelopes
Heparin Extraction of Nuclear Envelopes

Isolation of Protein Complexes
Multi-well Interactomic Screen
Affinity proteomics to study endogenous protein complexes
Conjugation of Tosylactivated Dynabeads
Conjugation of Magnetic Beads
Pullouts with IgG Resin
Immunoaffinity purifications of protein complexes

Mass Spectrometry
MALDI Sample Preparation_In-gel Digestion and Crystallization
MALDI Sample Preparation_The Ultra Thin Layer Method
MALDI Sample Preparation_Saturated 4-HCCA Solution
IMAC phosphopeptide enrichment
Phosphopeptide Enrichment Using TiO2 GL Biosciences

Genomic Analyses
mRNA Expression Microarrays
Agilent Expression Microarrays
ChIP (chromatin immunoprecipitation) for ChIP-CHIP analysis
Yeast Quantitative PCR Protocol

Protein Biochemistry
Carbonate Silver Stain
Electropurification
Methanol Precipitation of Protein
Protease Mapping
Strip Blots
Whole Cell Lysis and TCA (Trichloroacetic acid) Precipitation

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