Protocols

Below is a working list of NCDIR protocols and methodologies. Please contact us if you have questions or comments, or would like to submit a protocol. Disruption of Yeast or Bacteria Cells Harvesting Cells and Making Noodles Harvesting Cells and Making Noodles Cryogenic Disruption Using PM100 Ball Mill – grinding.mp4 Cryogenic Disruption of Yeast Cells PM100 Microfluidizer Zymolase Treatment Disruption of Mammalian Cells Cryogenic Disruption of Mammalian Cells using Retsch PM 100 Protein Affinity Capture from Cryogenically Disrupted Human Cells Biotechniques Paper Subcellular Fractionation Yeast Nuclear Envelopes and Nuclear Pore Complex Prep Yeast Nuclei & Spindle Pole Bodies Prep Carbonate Extraction of Nuclear Envelopes Rapid Subcellular Fractionation for Nuclei and Nuclear Envelopes Heparin Extraction of Nuclear Envelopes Isolation of Protein Complexes Multi-well Interactomic Screen Affinity proteomics to study endogenous protein complexes Conjugation of Tosylactivated Dynabeads Conjugation of Magnetic Beads Pullouts with IgG Resin Immunoaffinity purifications of protein complexes Mass Spectrometry MALDI Sample Preparation_In-gel Digestion and Crystallization MALDI Sample Preparation_The Ultra Thin Layer Method MALDI Sample Preparation_Saturated 4-HCCA Solution Peptide Mass Spectrometry IMAC phosphopeptide enrichment Phosphopeptide Enrichment Using TiO2 GL Biosciences Genomic Analyses mRNA Expression Microarrays Agilent Expression Microarrays ChIP (chromatin immunoprecipitation) for ChIP-CHIP analysis Yeast Quantitative PCR Protocol Protein Biochemistry Carbonate Silver Stain Electropurification Methanol Precipitation of Protein Protease Mapping Strip Blots Whole Cell Lysis and TCA (Trichloroacetic acid) Precipitation

biomedical-science-resources-400