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Latest News from the NCDIR

1201, 2023

Research Spotlight – A quantitative map of nuclear pore assembly reveals two distinct mechanisms

January 12, 2023|

The nuclear pore complex (NPC) consists of nearly ~1000 protein of ~30 different types, and controls macromolecular transport between the nucleus and the cytoplasm. Extensive experimental efforts and integrative modeling have provided significant insight into the static structure of this complex, but molecular details underlying how this complex assembles has been lacking. An improved understanding of the two possible NPC assembly pathways may elucidate the crucial role the NPC has played in eukaryotic evolution and eventually decode the link between genetic alterations in NPC associated proteins and a variety of human diseases. However, the size of the NPC complex and timescales approaching one hour for assembly completion have posed challenges to previous studies.

In recent work, researchers from the labs of Jan Ellenberg and Andrej Sali worked together to investigate the molecular mechanism of NPC assembly. Using fluorescence correlation spectroscopy calibrated live imaging, protein composition is tracked throughout the assembly process. This stoichiometric analysis sufficiently distinguished the two known pathways for NPC assembly, and revealed that these pathways proceed through different mechanisms. Specifically, the order in which the central ring complex and nuclear filaments are inverted.

To gain more insight into the NPC assembly mechanism, the dynamic stoichiometry data and prior electron tomographs of assembly intermediates were integrated to create a molecular model for one of the assembly pathways, specifically the postmitotic assembly pathway. This spatiotemporal model predicts the structure of NPC intermediates throughout the assembly process, allowing for mechanistic predictions into postmitotic assembly. For example, our model suggests that the central channel complex likely helps dilate the membrane pore throughout the assembly process.

Integrative model of the postmitotic NPC assembly pathway. a, Protein density (grey) overlaid with the nuclear envelope surface (wireframe model, grey) at each time point used for integrative modelling. C, cytoplasm; N, nucleoplasm. b, The best-scoring model of the postmitotic assembly pathway (top and side views). The Y-complex is shown in green; an isolated fraction of Nup155 that is not forming a complex with Nup93, Nup188 and Nup205 is shown in orange; Nup93–Nup188–Nup155 and Nup205–Nup93–Nup155 complexes are in blue; and the Nup62–Nup58–Nup54 complex is in purple. c, The enlargement of one of the spokes.

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3010, 2022

Research spotlight: “Viral protein engagement of GBF1 induces host cell vulnerability through synthetic lethality”

October 30, 2022|

“A dearth or antivirals”  Our current repertoire of U.S. Food and Drug Administration (FDA)-approved antivirals is limited to only nine out of the known 214 human-infecting RNA viruses, and almost all these antivirals target viral proteins. Unlike antivirals that target viral proteins, targeting host cell proteins can disrupt the ability of a virus to replicate, effectively shutting down the host cell “virus production facility”. An ideal host-based antiviral would selectively affect only virally infected cells, while leaving uninfected cells unscathed. But how could this work?

The key is that virally infected cells are different than the uninfected cells.

In infected cells, host cell proteins are called to action by the virus to ensure viral replication. In this process, they often leave their normal post, so their normal functions are compromised.  We call this a viral-induced hypomorph, which is analogous to a loss-of-function mutation. In cancer, where cells have cancer driver loss-of-function mutations, it is possible to selectively kill cancer cells with drugs that target proteins that are redundant in function to the mutated gene/protein. This is called ‘synthetic lethality’.

Synthetic lethality is a type of genetic interaction between two nonessential genes that participate in a parallel or redundant process to carry out an essential function, where mutations in either gene alone does not affect cell viability, but mutations in both genes result in cell death. We hypothesized that synthetic lethal (SL) interactions of viral-induced hypomorphs could be targeted as host-based antiviral therapeutics – selectively disrupting the viral production facility but leaving uninfected cells unaffected.

Proof of Concept GBF1, a Golgi membrane protein is a critical host factor for many RNA viruses including poliovirus, Coxsackievirus, Dengue, Hepatitis C and E virus, and Ebola virus. GBF1 becomes a hypomorph upon interaction with the poliovirus protein 3A. We performed a genome-wide chemogenomic CRISPR screen using a specific inhibitor of GBF1 called Golgicide A (GCA) in Nalm-6 cells to identify synthetic lethal partners of GBF1 and revealed ARF1 as the top hit. We showed that disruption of ARF1, selectively kills cells that synthesize poliovirus 3A alone or in the context of a poliovirus replicon. Consistent with our hypothesis, combining 3A expression with sub-lethal amounts of GCA further exacerbated the GBF1ARF1 SL effect.

Extending the principle of synthetic lethal interactions to a virus-induced hypomorph. (A) Synthetic lethality is an extreme negative genetic interaction occurring between two genes. Here, genes ‘A’ and ‘B’ are not essential, and the cell remains viable upon the loss of either gene, depicted by red dotted outline of ‘A’ or ‘B’, individually. However, when these deletions are combined in a single cell, this double loss of function critically impairs the cell, resulting in its death. Such gene-gene combinations are termed synthetic lethal (SL) partners. (B) In the cancerous cell, gene ‘A’ has been mutated, depicted as ‘A*’, leading to an enhanced dependency by the cancer cell for its synthetic lethal partner ‘B’. Drugs that target the otherwise nonessential gene B induce cell death when combined with its SL partner, A*. Therefore, inhibiting the function of B can selectively kill cancerous cells while sparing noncancerous bystander cells. (C) A viral infection provides opportunities for specifically targeting infected cells by synthetic lethality. In an infected cell, host factors, depicted as the red letter ‘A’, are recruited by viral proteins to support viral reproduction. The normal function of the host factor is thus attenuated by the presence of the virus, inducing a hypomorph, red letter ‘A’, which sensitizes the infected cell to inhibition of its synthetic lethal partner by an inhibitory drug.

The 3A induced hypomorph of GBF1 sensitizes cells to ARF1-GBF1 synthetic lethality. (A-C) SL killing of a vi-hypomorph of GBF1 by poliovirus protein 3A. (A) ARF1 and MSMO1 were stably silenced in HeLa cells and transfected with FLAG*-tagged poliovirus 3A. Cell viabilities of ARF1 KD and MSMO1 KD cells transfected with 3A-FLAG* or an empty plasmid control were measured with CellTiterBlue. (B) Single cell population (Side scatter (y-axis) positively stained with R-phycoerythrin (PE)-conjugated α-FLAG (x-axis) was quantified to measure the number of cells positive for 3A-FLAG* in ARF1 KD and MSMO1 KD cells by flow cytometry, post 24 h. (C) Poliovirus 3A induces cell death in ARF1 KD cells. Percent viabilities of ARF1 KD and MSMO1 KD cells treated with 3A-alone or GCA-alone (1.5 mM) and a combination of 3A and GCA were measured using CellTiterBlue. A multiple unpaired t-test was used to compare percent viability between the 3A-treated ARF1 KD and MSMO1 KD cells with **** representing P-value < 0.000001. (D-F) SL killing of a vi-hypomorph of GBF1 by poliovirus replicon. (D) HeLa cells were transiently transfected with siRNAs targeting ARF1 and a nontargeting control. The KD or control cells were transfected with 25 ng of poliovirus replicon mRNA, and cells were incubated for 24 h. During the time course of replicon transfection, cells were harvested and fixed at 8, 16, and 24 h, immunostained, and imaged by fluorescence microscopy. (E) Representative immunofluorescence images of nontargeting and ARF1 KD cells transfected with poliovirus replicon or mock. DAPI (cyan) was used to stain cell nuclei and the viral 3A-FLAG* protein was immunostained with a primary antibody against the FLAG tag (orange) (F) Quantification of cell depletion. DAPI signal was used to count the total number of imaged cells. Multiple t-test was used to compare cell death between poliovirus replicon-transfected ARF1 KD and nontargeting controls at each time point. Statistically significant differences were observed at 8 h (P-value < 0.05 indicated as *) and at 16 h (P-value < 0.01, indicated as **).

The Future As viruses co-opt common host factors, SL-derived antivirals have a potential to be broad-spectrum. We established that viral protein interactions can induce hypomorphs that render host cells selectively vulnerable to perturbations that leave uninfected cells otherwise unscathed. Our strategy to target SL interactions of the viral-induced hypomorph has the potential to change the current paradigm for host-based therapeutics that can lead to broad-spectrum antivirals and can be applied to other intracellular pathogens.

Funding agencies:

This work is supported by National Institutes of Health grants R01 GM112108 and P41 GM109824, R21 AI151344 and foundation grant FDN-167277 from the Canadian Institutes of Health Research.

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2510, 2022

The NCDIR’s John Aitchison awarded the Lung Association’s coveted COVID-19 Respiratory Virus Research Award

October 25, 2022|

Dr. John Aitchison

The NCDIR’s Dr. John Aitchison has been awarded the Lung Association’s  COVID-19 Respiratory Virus Research Award.

Dr. Aitchison’s  project is geared to further research on the use of inhaled nanobodies to complement current COVID-19 vaccines and drugs to serve critically ill patients, act as a preventative treatment and retain effectiveness new variants of the virus

“Here in Washington, we face continued lung health challenges from COVID-19 every day. This is in addition to our region’s lung health being threatened by poor air quality due to increased wildfire smoke, continued cigarette and e-cigarette use and more. Today, more than 917,000 people in our state are living with chronic lung disease…We are excited for John Aitchison to join the American Lung Association Research Team to help improve lung health here in Washington and across the nation.”  – Season Oltmann, Executive Director of the American Lung Association

Read more here: Lung.org

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